Journal: Pharmaceutics
Article Title: Intestinal Cellular Biomarkers of Mucosal Lesion Progression in Pediatric Celiac Disease
doi: 10.3390/pharmaceutics13111971
Figure Lengend Snippet: Correlation between the frequency of TCRγδ+ T cells and IL4+ T cells in potential- and overt-CD patients. ( a ) The percentages of TCRγδ+ T cells and IL4+ T cells were measured in duodenal mucosa samples of 41 children enrolled in the study ( n = 12 with overt-CD, 18 with potential-CD and 11 non-CD controls). ( b ) The densities of CD3+ and TCRγδ+ cells were analyzed by a multiparametric flow cytometry in unstimulated condition, whereas the INF-γ and IL4 production was assessed without ( c ) or with PMA/Ionomycin 3 h of stimulation ( d , e ) both on freshly isolated intestinal cells and on short-term CD3+ cell lines (st-TCLs), as detailed in the materials and methods. Each symbol indicates the cell percentages detected in each subject. The correlation was assessed using the non-parametric Spearman’s rank correlation coefficient by GraphPad Prism Software, with a p ≤ 0.05 considered statistically significant. Representative flow cytometry dot plots from one overt-CD (left panels of b – e ) and one potential-CD (right panels of b – e ) patient, showing the percentage of CD3+ and TCRγδ+ cells positive for IFNγ and IL4 production, are reported.
Article Snippet: Correlations between variables were assessed using the non-parametric Spearman’s rank correlation coefficient by GraphPad Prism Software, with a p ≤ 0.05 considered as statistically significant.
Techniques: Flow Cytometry, Isolation, Software